My research program focuses on understanding the cell and molecular basis of wound
healing. Our work has three foci: (1) Normal healing.
We study primarily the activation, expression, function
and mode of action of chemokines in healing, using animal models and complex human cultures. Our studies have
focused on the chemokine Interleukine-8 (IL-8), what agents at the wound site stimulate its expression, the
signal-transduction mechanisms by which this expression occurs, and what functions IL-8 performs during the
healing process. Our aim is to: (a
) Identify commonalities in the signal transduction and transcription
activation mechanisms that may lead the way to regulating the expression of chemokines for potential medical
) test the effects of IL-8 on the principal cellular components of the granulation tissue of
wounds -- fibroblasts, myofibroblasts, endothelial cells, keratinocytes and immune cells - accompanied by tests
directly to wounds; (c
) characterize the function of IL-8 receptor(s) during wound healing. (2) Impaired healing.
Effects of environmental toxicants, especially those present in cigarette smoke. We study the effects of
second-hand smoke on: (a
) the development of atherosclerosis (a form of impaired healing); (b
) delay of skin and
cornea wound healing. Our aim here is to identify key processes affected by cigarette smoke and which chemicals
in the smoke cause those effects. (3) Engineering of human tissues.
In order to test the relevance for human
biology of some of the hypotheses we have developed in animal systems, we are developing complex tissue cultures
using primary human cells. We have already developed a novel human "skin" organ culture that we are now using to
study some of the fundamental cell and molecular processes in normal and abnormal healing of skin. We have also
developed a system that allows us to test the effects of inflammatory agents on endothelial permeability and we
are currently developing an arterial wall model and a lung epithelial/endothelial blood barrier system for studies
of atherogenesis induced by first- and second-hand cigarette smoke.
Visit the Martins-Green Lab
See publications on PubMed
Hongwei Yuan, Lina Wong, Monideepa Bhattacharya, Matthias Schneider, Robert E. Pitas and
Manuela Martins-Green. Second-Hand Smoke Effects on Biological Processes Important in Atherogenesis. BMC
Cardiovascular Disease, in Press.
Dueck-Petreaca, Melissa and Martins-Green (2006). "Cell-Extracellular Matrix Interactions
with implications for Tissue engineering" in Principles of Tissue engineering, 3rd edition, edited by Robert Lanza,
Robert Langer and Joseph Vacanti.
Dueck-Petreaca, Melissa and Martins-Green (2006). "Biologic and Molecular Basis of
Regenerative Medicine: Cell-Extracellular Matrix Interactions" in Principles of Regenerative Medicine, edited
by Anthony Atala, Robert Lanza, James Thomson, Robert Nerem.
Yao, Min, Rui-Hai Zhou, Melissa Petreaca, John Shyy and Manuela Martins-Green (2006). "Signal
Transduction Pathways Stimulated by Different Types of Angiogenic Factors Converge in Activation of SREBPs and
RhoA". J. Leukocyte Biology 80(3): 608-20. Epub 2006 Jul 18.
Li, Qi-Jing, Min Yao, Melissa Dueck, Vladimir Parpura and Manuela Martins-Green (2005).
cCXCR1 is a Receptor for cIL8 (9E3/cCAF) and its N- and C-Terminal Peptides, and is also Activated by hIL8 (CXCL8).
J. Leukocyte Biology, 77:1-14.
Martins-Green, M. Q-J Li and Min Yao (2004). A new generation organ culture arising from
cross-talk between multiple primary human cell types. Epub 2004 Dec 9. PMID: 15591154.
Wong, L. and M. Martins-Green (2004). "Firsthand" cigarette smoke alters fibroblasts
migration and survival: Implications for impaired Healing. Wound Repair and Regeneration 12(4):471-84.